Coding

Part:BBa_M10034:Design

Designed by: Sadao Ota   Group: Berkeley BioE140L - S09   (2009-05-10)

{˂Ag43_short!}, for Ag 43 autotransporter display system, only transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Bglbricking of M10034, Ag43 (autotransporter only) PCR Oso001 and Oso002 on E. coli MG1655 genomic DNA (937 bp, EcoRI/BamHI) Sub into pBca9495KC-Bca1144#5 (EcoRI/BamHI, L) Product is pBca9495KC-M10034 {<Ag43_short!}

Oso001 Cloning of Ag43, short ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg

Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg


Source

The source of this part can be found from Escherichia coli str. K-12 substr. MG1655, complete genome.

References